Structural and functional investigation of tRNA guanine transglycosylase

Katharina Sievers
University of Göttingen Dissertation - (2023 Nov 17)

URL: https://dx.doi.org/10.53846/goediss-10207

Submitted to SASBDB: 2023 Apr 3 Published in SASBDB: 2023 Dec 15

SASDRB8 – Human tRNA guanine transglycosylase heterodimer Sample: Queuine tRNA-ribosyltransferase catalytic subunit 1 monomer, 44 kDa Homo sapiens protein Queuine tRNA-ribosyltransferase accessory subunit 2 monomer, 47 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1 RgGuinier 3.3 nm Dmax 11.4 nm VolumePorod 127 nm3

SASDRC8 – Human tRNA guanine transglycosylase bound to aspartate transfer RNA (tRNA-Asp) Sample: Queuine tRNA-ribosyltransferase catalytic subunit 1 monomer, 44 kDa Homo sapiens protein Queuine tRNA-ribosyltransferase accessory subunit 2 monomer, 47 kDa Homo sapiens protein Transfer RNA (Aspartate) monomer, 25 kDa Homo sapiens RNA Buffer: 20 mM HEPES, 100 mM NaCl, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1 RgGuinier 3.5 nm Dmax 10.9 nm VolumePorod 160 nm3