I-Shaped Dimers of a Plant Chloroplast FOF1-ATP Synthase in Response to Changes in Ionic Strength

Osipov S, Ryzhykau Y, Zinovev E, Minaeva A, Ivashchenko S, Verteletskiy D, Sudarev V, Kuklina D, Nikolaev M, Semenov Y, Zagryadskaya Y, Okhrimenko I, Gette M, Dronova E, Shishkin A, Dencher N, Kuklin A, Ivanovich V, Uversky V, Vlasov A
International Journal of Molecular Sciences 24(13):10720 (2023 Jun 27)

doi: 10.3390/ijms241310720

Submitted to SASBDB: 2023 Apr 17 Published in SASBDB: 2023 Jun 27

SASDRR8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea purified by anion-exchange chromatography (~ 300 mM NaCl) Sample: ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa Buffer: 300 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3 RgGuinier 6.6 nm Dmax 27.5 nm VolumePorod 927 nm3

SASDRS8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 150 mM NaCl Sample: ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa Buffer: 150 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3 RgGuinier 9.6 nm Dmax 41.5 nm VolumePorod 1506 nm3

SASDRT8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 250 mM NaCl Sample: ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa Buffer: 250 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3 RgGuinier 7.4 nm Dmax 33.0 nm VolumePorod 949 nm3

SASDRU8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 300 mM NaCl Sample: ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa Buffer: 300 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3 RgGuinier 7.8 nm Dmax 39.5 nm VolumePorod 1127 nm3

SASDRV8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 350 mM NaCl Sample: ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa Buffer: 350 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3 RgGuinier 8.9 nm Dmax 44.5 nm VolumePorod 1150 nm3

SASDRW8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 450 mM NaCl Sample: ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa Buffer: 450 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3 RgGuinier 10.8 nm Dmax 46.5 nm VolumePorod 1703 nm3