Crystal structure and activity of a de novo enzyme, ferric enterobactin esterase Syn-F4.

Kurihara K, Umezawa K, Donnelly AE, Sperling B, Liao G, Hecht MH, Arai R
Proc Natl Acad Sci U S A 120(38):e2218281120 (2023 Sep 19)
PMID: 37695900
doi: 10.1073/pnas.2218281120 		Submitted to SASBDB: 2023 Apr 7
Published in SASBDB:

SASDRJ8 – A de novo enzyme, ferric enterobactin esterase Syn-F4

De novo enterobactin esterase Syn-F4 (K4T) experimental SAS data
DAMMIN model
Sample: De novo enterobactin esterase Syn-F4 (K4T) dimer, 25 kDa synthetic construct protein
Buffer: 25 mM MES, 100 mM NaCl, 10% glycerol, 200 mM Arg-HCl, pH: 6.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 Feb 11
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 40 nm3