Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors.

Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
Protein J (2023 Sep 20)

PMID: 37728788 doi: 10.1007/s10930-023-10156-y

Submitted to SASBDB: 2023 May 8 Published in SASBDB: 2023 Sep 22

SASDSZ4 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) unliganded form Sample: Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 3.3 nm Dmax 9.9 nm VolumePorod 153 nm3

SASDS25 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Nevirapine Sample: Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 3.5 nm Dmax 10.9 nm VolumePorod 161 nm3

SASDS35 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) unliganded form Sample: Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 3.1 nm Dmax 10.7 nm VolumePorod 98 nm3

SASDS45 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Efavirenz Sample: Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 3.7 nm Dmax 11.3 nm VolumePorod 178 nm3

SASDS55 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Nevirapine Sample: Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 3.5 nm Dmax 11.3 nm VolumePorod 134 nm3

SASDS65 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Efavirenz Sample: Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 4.6 nm Dmax 15.0 nm VolumePorod 270 nm3

SASDS75 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) unliganded form Sample: Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 2.8 nm Dmax 9.8 nm VolumePorod 84 nm3

SASDS85 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Nevirapine Sample: Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 3.1 nm Dmax 11.1 nm VolumePorod 103 nm3

SASDS95 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Efavirenz Sample: Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19 RgGuinier 4.2 nm Dmax 16.7 nm VolumePorod 226 nm3