Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: implication in AADC deficiency

Bisello G, Ribeiro R, Perduca M, Belviso B, Polverino de' Laureto P, Giorgetti A, Caliandro R, Bertoldi M
Protein Science (2023 Jul 19)

doi: 10.1002/pro.4732 		Submitted to SASBDB: 2023 Apr 14
Published in SASBDB:

SASDR89 – Aromatic-L-amino-acid decarboxylase (AADC) - apo form

Aromatic-L-amino-acid decarboxylase (M17V) experimental SAS data
DAMMIN model
Sample: Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Feb 28
RgGuinier 3.4 nm
Dmax 15.8 nm
VolumePorod 181 nm3

SASDR99 – Aromatic-L-amino-acid decarboxylase (AADC) - pyridoxal 5'-phosphate bound holo form

Aromatic-L-amino-acid decarboxylase (M17V) experimental SAS data
DAMMIN model
Sample: Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Feb 28
RgGuinier 3.2 nm
Dmax 14.9 nm
VolumePorod 180 nm3

SASDRA9 – Aromatic-L-amino-acid decarboxylase (AADC) - dopa methylester DME-bound holo form

Aromatic-L-amino-acid decarboxylase (M17V) experimental SAS data
DAMMIN model
Sample: Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Feb 28
RgGuinier 3.0 nm
Dmax 14.5 nm
VolumePorod 154 nm3