The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with CXCL12.

Mantonico MV, De Leo F, Quilici G, Colley LS, De Marchis F, Crippa M, Mezzapelle R, Schulte T, Zucchelli C, Pastorello C, Carmeno C, Caprioglio F, Ricagno S, Giachin G, Ghitti M, Bianchi ME, Musco G
Nat Commun 15(1):1201 (2024 Feb 8)

PMID: 38331917 doi: 10.1038/s41467-024-45505-7

Submitted to SASBDB: 2023 Apr 21 Published in SASBDB: 2023 Jun 27

SASDRG9 – Stromal cell-derived factor 1, CXCL12 Sample: Stromal cell-derived factor 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 11 RgGuinier 1.5 nm Dmax 4.8 nm VolumePorod 19 nm3

SASDRH9 – High mobility group protein B1, HMGB1 Sample: High mobility group protein B1 (D189E, E202D, E215D) monomer, 25 kDa Rattus norvegicus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 11 RgGuinier 2.6 nm Dmax 8.5 nm VolumePorod 53 nm3

SASDRJ9 – High mobility group protein B1/Stromal cell-derived factor 1 complex (HMGB1-CXCL12) Sample: Stromal cell-derived factor 1 monomer, 9 kDa Homo sapiens protein High mobility group protein B1 (D189E, E202D, E215D) monomer, 25 kDa Rattus norvegicus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 11 RgGuinier 2.9 nm Dmax 10.5 nm VolumePorod 72 nm3