The solution structure of the heavy chain-only C5-Fc nanobody reveals exposed variable regions that are optimal for COVID-19 antigen interactions.

Gao X, Thrush JW, Gor J, Naismith JH, Owens RJ, Perkins SJ
J Biol Chem :105337 (2023 Oct 12)
PMID: 37838175
doi: 10.1016/j.jbc.2023.105337 		Submitted to SASBDB: 2023 Apr 28
Published in SASBDB:

SASDSD2 – Recombinant Heavy-chain-only nanobody C5-Fc (glycosylated)

Immunoglobulin heavy constant gamma 1 experimental SAS data
PYMOL model
Sample: Immunoglobulin heavy constant gamma 1 monomer, 78 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Sep 28
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 157 nm3

SASDSE2 – Recombinant Heavy-chain-only nanobody C5-Fc (Deglycosylated)

Immunoglobulin heavy constant gamma 1 experimental SAS data
PYMOL model
Sample: Immunoglobulin heavy constant gamma 1 monomer, 78 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Sep 28
RgGuinier 4.0 nm
Dmax 13.1 nm
VolumePorod 175 nm3