Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles

Ehrit J, Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N
Biophysical Journal (2023 Jun)

doi: 10.1016/j.bpj.2023.06.011

Submitted to SASBDB: 2023 Apr 24 Published in SASBDB: 2023 Jul 4

SASDRN9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) Sample: Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 0, 45 kDa Buffer: aqueous solution of 4% methanol, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 2 RgGuinier 3.4 nm Dmax 8.6 nm

SASDRQ9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 20-mer, 24 kDa Buffer: aqueous solution of 4% methanol, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6 RgGuinier 3.0 nm Dmax 8.0 nm

SASDRS9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 0, 42 kDa Buffer: aqueous solution of 4% methanol, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 29 RgGuinier 3.3 nm Dmax 8.3 nm

SASDRP9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) Sample: Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 0, 45 kDa Buffer: aqueous solution of 4% methanol, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 2 RgGuinier 3.5 nm Dmax 8.8 nm

SASDRR9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 22-mer, 26 kDa Buffer: aqueous solution of 4% methanol, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6 RgGuinier 3.1 nm Dmax 8.3 nm

SASDRT9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 0, 42 kDa Buffer: aqueous solution of 4% methanol, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 29 RgGuinier 3.5 nm Dmax 8.7 nm