Structural and biochemical characterization of the C‐terminal region of the human RTEL 1 helicase

Cortone G, Graewert M, Kanade M, Longo A, Hegde R, González‐Magaña A, Chaves‐Arquero B, Blanco F, Napolitano L, Onesti S
Protein Science 33(9) (2024 Aug 24)

doi: 10.1002/pro.5093

Submitted to SASBDB: 2023 Apr 27 Published in SASBDB: 2024 Aug 26

SASDRZ9 – Regulator of telomere elongation helicase 1, HHD-1 domain Sample: Regulator of telomere elongation helicase 1 (Isoform 6, 889-974) monomer, 10 kDa Homo sapiens protein Buffer: 50 mM Tris, 150 mM NaCl,10 (v/v)% glycerol, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 22 RgGuinier 1.5 nm Dmax 4.6 nm VolumePorod 22 nm3

SASDRY9 – Regulator of telomere elongation helicase 1, HHD-2 domain Sample: Regulator of telomere elongation helicase 1 (Isoform 6, 1053-1147) monomer, 11 kDa Homo sapiens protein Buffer: 50 mM Hepes, 150 mM NaCl,10 (v/v)% glycerol, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 22 RgGuinier 1.5 nm Dmax 5.0 nm VolumePorod 24 nm3

SASDRW9 – Regulator of telomere elongation helicase 1, HHD1-HHD2 fragment Sample: Regulator of telomere elongation helicase 1 (Isoform 6, 889-1147) monomer, 28 kDa Homo sapiens protein Buffer: 50 mM Hepes, 150 mM NaCl,10 (v/v)% glycerol, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 22 RgGuinier 3.4 nm Dmax 10.1 nm VolumePorod 36 nm3

SASDRX9 – Regulator of telomere elongation helicase 1, C - terminal, CT-S fragment Sample: Regulator of telomere elongation helicase 1 (Isoform 6, 756-1219) monomer, 50 kDa Homo sapiens protein Buffer: 50 mM Hepes, 150 mM NaCl,10 (v/v)% glycerol, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 22 RgGuinier 4.9 nm Dmax 18.0 nm VolumePorod 121 nm3

SASDRV9 – Regulator of telomere elongation helicase 1, C - terminal Sample: Regulator of telomere elongation helicase 1 (Isoform 6, 756-1300) monomer, 59 kDa Homo sapiens protein Buffer: 50 mM Hepes, 150 mM NaCl,10 (v/v)% glycerol, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 22 RgGuinier 4.9 nm Dmax 15.3 nm VolumePorod 132 nm3