Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes.

Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C
Genes Dev 37(11-12):535-553 (2023 Jun 1)
PMID: 37442581
doi: 10.1101/gad.350462.123 		Submitted to SASBDB: 2023 May 19
Published in SASBDB:

SASDSP2 – Saccharomyces cerevisiae Mer2 coiled-coiled with N-terminal SUMO-tag

Recombination protein 107 experimental SAS data
Sample: Recombination protein 107 tetramer, 139 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
RgGuinier 8.3 nm
Dmax 32.2 nm

SASDSU2 – Mus musculus Iho1 coiled-coiled with N-terminal SUMO-tag

Interactor of HORMAD1 protein 1 (M198V, V230I) experimental SAS data
Sample: Interactor of HORMAD1 protein 1 (M198V, V230I) tetramer, 127 kDa Mus musculus protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
RgGuinier 7.2 nm
Dmax 25.5 nm

SASDST2 – Saccharomyces pombe Rec15 coiled-coiled with N-terminal SUMO-tag

Meiotic recombination protein rec15 experimental SAS data
3D-DART model
Sample: Meiotic recombination protein rec15 tetramer, 128 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
RgGuinier 6.9 nm
Dmax 26.5 nm

SASDSS2 – Arabidopsis thaliana PRD3 coiled-coiled with N-terminal SUMO-tag

Putative recombination initiation defects 3 experimental SAS data
OTHER model
Sample: Putative recombination initiation defects 3 tetramer, 129 kDa Arabidopsis thaliana protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
RgGuinier 7.8 nm
Dmax 27.5 nm

SASDSR2 – Zea mays PAIR1 coiled-coiled with N-terminal SUMO-tag

Protein PAIR1 experimental SAS data
OTHER model
Sample: Protein PAIR1 tetramer, 132 kDa Zea mays protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
RgGuinier 8.0 nm
Dmax 31.7 nm

SASDSQ2 – Sordaria macrospora ASY2 coiled-coiled with N-terminal SUMO-tag

WGS project CABT00000000 data, contig 2.12 experimental SAS data
OTHER model
Sample: WGS project CABT00000000 data, contig 2.12 tetramer, 155 kDa Sordaria macrospora (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
RgGuinier 9.6 nm
Dmax 38.0 nm