Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose).

Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
Nat Commun 15(1):7901 (2024 Sep 10)

PMID: 39256374 doi: 10.1038/s41467-024-51972-9

Submitted to SASBDB: 2023 May 30 Published in SASBDB: 2023 Nov 15

SASDSJ5 – 15mer poly-ADP ribose (PAR15) in 100mM NaCl Sample: 15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct Buffer: 100 mM NaCl, 20 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21 RgGuinier 2.4 nm Dmax 10.0 nm VolumePorod 15 nm3

SASDSK5 – 15mer poly-ADP ribose (PAR15) in 100mM NaCl + 1mM MgCl2 Sample: 15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct Buffer: 20 mM Tris-HCl, 1 mM MgCl2, 100 mM NaCl, pH: 7.4 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21 RgGuinier 2.3 nm Dmax 9.0 nm VolumePorod 14 nm3

SASDSL5 – 22mer poly-ADP ribose (PAR22) in 100mM NaCl Sample: 22mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 12 kDa synthetic construct Buffer: 100 mM NaCl, 20 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Apr 21 RgGuinier 3.3 nm Dmax 14.0 nm VolumePorod 32 nm3

SASDSM5 – 22mer poly-ADP ribose (PAR22) in 100mM NaCl + 1mM MgCl2 Sample: 22mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 12 kDa synthetic construct Buffer: 20 mM Tris-HCl, 1 mM MgCl2, 100 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Apr 21 RgGuinier 2.6 nm Dmax 10.5 nm VolumePorod 21 nm3