TIA-1 RRM23 binding and recognition of target oligonucleotides.

Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA
Nucleic Acids Res 45(8):4944-4957 (2017 May 5)

PMID: 28184449 doi: 10.1093/nar/gkx102

Submitted to SASBDB: 2016 Sep 22 Published in SASBDB: 2017 Mar 28

SASDBM6 – Nucleolysin TIA-1 isoform p40 Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Jul 2 RgGuinier 2.3 nm Dmax 8.8 nm VolumePorod 26 nm3

SASDBR6 – Nucleolysin TIA-1 isoform p40 in complex with UUUUUACUCC RNA Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein RNA (UUUUUACU) monomer, 1 kDa RNA Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27 RgGuinier 2.1 nm Dmax 6.5 nm VolumePorod 26 nm3

SASDBQ6 – Nucleolysin TIA-1 isoform p40 in complex with TTTTTACTCC DNA Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein DNA (TTTTTACTCC) monomer, 1 kDa DNA Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27 RgGuinier 2.1 nm Dmax 7.1 nm VolumePorod 32 nm3

SASDBN6 – Nucleolysin TIA-1 isoform p40 in complex with ACUCCUUUUU RNA Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein RNA (ACUCCUUUUU) monomer, 1 kDa RNA Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27 RgGuinier 2.2 nm Dmax 6.6 nm VolumePorod 30 nm3

SASDBP6 – Nucleolysin TIA-1 isoform p40 in complex with ACTCCTTTTT DNA Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein DNA (ACTCCTTTTT) monomer, 1 kDa DNA Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27 RgGuinier 2.2 nm Dmax 6.7 nm VolumePorod 32 nm3