SEC-SAXS profiles of ERalpha-NTD: wild-type, mutant, and phosphorylated Ser118 variants

Sichun Yang

Submitted to SASBDB: 2024 Jul 23 Published in SASBDB: 2024 Dec 13

SASDSK2 – SEC-SAXS data of wild-type estrogen receptor alpha, N-terminal domain (NTD) at pH 7.4 Sample: Estrogen receptor monomer, 20 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 150 mM NaCl, 0.5 mM EDTA, 0.1 mM PMSF, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jul 21 RgGuinier 3.2 nm Dmax 14.0 nm VolumePorod 47 nm3

SASDVC5 – SEC-SAXS data of phosphorylated estrogen receptor alpha (pSer118), N-terminal domain (NTD) at pH 7.4 Sample: Estrogen receptor monomer, 20 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH: 7.4 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jul 17 RgGuinier 3.6 nm Dmax 16.0 nm VolumePorod 70 nm3

SASDSE5 – SEC-SAXS data of mutant estrogen receptor alpha (S118A), N-terminal domain (NTD) at pH 7.4 Sample: Estrogen receptor (mutant S118A) monomer, 20 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 150 mM NaCl, 0.5 mM EDTA, 0.1 mM PMSF, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jul 21 RgGuinier 3.2 nm Dmax 15.0 nm VolumePorod 71 nm3

SASDSF5 – SEC-SAXS data of mutant estrogen receptor alpha (S118D), N-terminal domain (NTD) at pH 7.4 Sample: Estrogen receptor (mutant S118D) monomer, 20 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 150 mM NaCl, 0.5 mM EDTA, 0.1 mM PMSF, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jul 21 RgGuinier 3.5 nm Dmax 16.0 nm VolumePorod 62 nm3