The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease.

Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
Nat Commun 14(1):5625 (2023 Sep 12)

PMID: 37699927 doi: 10.1038/s41467-023-40023-4

Submitted to SASBDB: 2023 Jun 1 Published in SASBDB: 2023 Jun 28

SASDSP5 – SARS-CoV-2 Main Protease H163A Mutant - 0.25 mg/mL Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24 RgGuinier 2.4 nm Dmax 7.8 nm VolumePorod 63 nm3

SASDSQ5 – SARS-CoV-2 Main Protease H163A Mutant - 0.5 mg/mL Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24 RgGuinier 2.5 nm Dmax 8.2 nm VolumePorod 74 nm3

SASDSR5 – SARS-CoV-2 Main Protease H163A Mutant - 1.0 mg/mL Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24 RgGuinier 2.6 nm Dmax 8.2 nm VolumePorod 85 nm3

SASDSS5 – SARS-CoV-2 Main Protease H163A Mutant - 3.0 mg/mL Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24 RgGuinier 2.6 nm Dmax 8.5 nm VolumePorod 102 nm3

SASDST5 – SARS-CoV-2 Main Protease H163A Mutant - 6.3 mg/mL Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24 RgGuinier 2.6 nm Dmax 7.7 nm VolumePorod 98 nm3