Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae.

Hadži S, Živič Z, Kovačič M, Zavrtanik U, Haeserts S, Charlier D, Plavec J, Volkov AN, Lah J, Loris R
Nat Commun 15(1):3105 (2024 Apr 10)
PMID: 38600130
doi: 10.1038/s41467-024-47296-3
Submitted to SASBDB: 2023 Jun 21
Published in SASBDB:

SASDS76 – Antitoxin HigA-2

Antitoxin HigA-2 experimental SAS data
Antitoxin HigA-2 Kratky plot
Sample: Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris pH 8, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Apr 6
RgGuinier 2.3 nm
Dmax 9.6 nm
VolumePorod 43 nm3

SASDS86 – Antitoxin HigA-2 in complex with a DNA operator sequence

Antitoxin HigA-2DNA operator experimental SAS data
Antitoxin HigA-2 DNA operator Kratky plot
Sample: Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein
DNA operator dimer, 20 kDa DNA
Buffer: 20 mM Tris pH 8, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Apr 6
RgGuinier 2.7 nm
Dmax 9.6 nm
VolumePorod 57 nm3