Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity.

Bárria C, Athayde D, Hernandez G, Fonseca L, Casinhas J, Cordeiro TN, Archer M, Arraiano CM, Brito JA, Matos RG
Biochimie (2023 Oct 6)
PMID: 37806617
doi: 10.1016/j.biochi.2023.10.006 		Submitted to SASBDB: 2023 Jun 26
Published in SASBDB:

SASDLC7 – Polyribonucleotide nucleotidyltransferase (PNPase): apo form

Polyribonucleotide nucleotidyltransferase experimental SAS data
DAMFILT model
Sample: Polyribonucleotide nucleotidyltransferase trimer, 246 kDa Campylobacter jejuni subsp. … protein
Buffer: 20 mM Tris-HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 17
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 310 nm3

SASDLD7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-AMP complex

Polyribonucleotide nucleotidyltransferase experimental SAS data
DAMFILT model
Sample: Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer: 20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 17
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 315 nm3

SASDLE7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-GMP complex

Polyribonucleotide nucleotidyltransferase experimental SAS data
DAMFILT model
Sample: Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer: 20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1mM MgCl2, 2 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 17
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 312 nm3