Structural and dynamic changes in P-Rex1 upon activation by PIP3 and inhibition by IP4

Ravala S, Adame-Garcia S, Li S, Chen C, Cianfrocco M, Silvio Gutkind J, Cash J, Tesmer J
eLife 12 (2024 Jul 31)

doi: 10.7554/eLife.92822.4 		Submitted to SASBDB: 2024 Jan 26
Published in SASBDB:

SASDUF2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (P-Rex1-DH/PH-DEP1 (WT))

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 8
RgGuinier 3.0 nm
Dmax 9.7 nm
VolumePorod 79 nm3

SASDUG2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein A170K mutant (P-Rex1-DH/PH-DEP1-A170K)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (A170K) experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (A170K) monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 24
RgGuinier 2.9 nm
Dmax 9.0 nm
VolumePorod 76 nm3

SASDUH2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein L177E mutant (P-Rex1-DH/PH-DEP1-L177E)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (L177E) experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (L177E) monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 8
RgGuinier 3.1 nm
Dmax 11.0 nm
VolumePorod 88 nm3

SASDUJ2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein I409A mutant (P-Rex1-DH/PH-DEP1-I409A)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (I409A) experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (I409A) monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 8
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 79 nm3