Intramolecular autoinhibition regulates the selectivity of PRPF40A tandem WW domains for proline-rich motifs.

Martínez-Lumbreras S, Träger LK, Mulorz MM, Payr M, Dikaya V, Hipp C, König J, Sattler M
Nat Commun 15(1):3888 (2024 May 8)

PMID: 38719828 doi: 10.1038/s41467-024-48004-x

Submitted to SASBDB: 2023 Jul 18 Published in SASBDB: 2024 Mar 26

SASDSK7 – Tandem of WW domains of Pre-mRNA-processing factor 40 homolog A (PRPF40A) Sample: Pre-mRNA-processing factor 40 homolog A monomer, 11 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 100 mM NaCl, 0.5 mM DTT, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Sep 19 RgGuinier 2.0 nm Dmax 6.4 nm VolumePorod 17 nm3

SASDSL7 – Tandem WW domains of Pre-mRNA-processing factor 40 homolog A (PRPF40A) in complex with Splicing factor 1 (SF1) C-terminal proline repeat Sample: Pre-mRNA-processing factor 40 homolog A monomer, 11 kDa Homo sapiens protein Splicing factor 1 monomer, 2 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 100 mM NaCl, 0.5 mM DTT, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Sep 19 RgGuinier 1.9 nm Dmax 6.9 nm VolumePorod 19 nm3

SASDUH4 – Tandem of WW domains of Pre-mRNA-processing factor 40 homolog A (PRPF40A) in HEPES buffer Sample: Pre-mRNA-processing factor 40 homolog A monomer, 11 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 6.6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, Technische Universität München on 2024 Mar 8 RgGuinier 2.0 nm Dmax 6.3 nm VolumePorod 18 nm3

SASDUJ4 – Tandem of WW domains of Pre-mRNA-processing factor 40 homolog A (PRPF40A) in complex with Splicing factor 1 (SF1) in HEPES buffer Sample: Pre-mRNA-processing factor 40 homolog A monomer, 11 kDa Homo sapiens protein Splicing factor 1 monomer, 2 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 6.6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, Technische Universität München on 2024 Mar 8 RgGuinier 1.9 nm Dmax 6.0 nm VolumePorod 19 nm3