14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices.

Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
Protein Sci :e4805 (2023 Oct 10)

PMID: 37817008 doi: 10.1002/pro.4805

Submitted to SASBDB: 2023 Aug 21 Published in SASBDB: 2023 Oct 24

SASDSJ7 – Human C-terminally truncated 14-3-3 gamma Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3 RgGuinier 2.8 nm Dmax 8.4 nm VolumePorod 76 nm3

SASDSR8 – Calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1) Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17 RgGuinier 3.2 nm Dmax 10.7 nm VolumePorod 114 nm3

SASDSS8 – Phosphorylated Calcium/calmodulin-dependent protein kinase 1 (pCaMKK1) Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17 RgGuinier 3.0 nm Dmax 10.5 nm VolumePorod 97 nm3

SASDSQ7 – Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) Sample: Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3 RgGuinier 3.2 nm Dmax 13.3 nm VolumePorod 91 nm3

SASDST8 – Phosphorylated Calcium/calmodulin-dependent protein kinase 2 (pCaMKK2) Sample: Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17 RgGuinier 3.0 nm Dmax 12.0 nm VolumePorod 87 nm3

SASDS46 – Human phosphorylated calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1) bound to 14-3-3 protein gamma Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23 RgGuinier 3.3 nm Dmax 10.8 nm VolumePorod 152 nm3

SASDSX7 – Human phosphorylated CaMKK2 bound to 14-3-3 gamma Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23 RgGuinier 3.7 nm Dmax 13.2 nm VolumePorod 157 nm3