Integrating molecular dynamics simulation with small- and wide-angle X-ray scattering to unravel the flexibility, antigen-blocking, and protease-restoring functions in a hindrance-based pro-antibody.

Liao JM, Hong ST, Wang YT, Cheng YA, Ho KW, Toh SI, Shih O, Jeng US, Lyu PC, Hu IC, Huang MY, Chang CY, Cheng TL
Protein Sci 33(9):e5124 (2024 Sep)
PMID: 39145427
doi: 10.1002/pro.5124 		Submitted to SASBDB: 2023 Aug 11
Published in SASBDB:

SASDSN8 – Pro-Nivolumab, Lu02

Pro-Nivolumab, Lu02 experimental SAS data
AMBER model
Sample: Pro-Nivolumab, Lu02 monomer, 54 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at TPS13A, NSRRC on 2022 Sep 8
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 69 nm3