Structural investigation of an RNA device that regulates PD-1 expression in mammalian cells.

Stagno JR, Deme JC, Dwivedi V, Lee YT, Lee HK, Yu P, Chen SY, Fan L, Degenhardt MFS, Chari R, Young HA, Lea SM, Wang YX
Nucleic Acids Res 53(5) (2025 Feb 27)
PMID: 40071935
doi: 10.1093/nar/gkaf156 		Submitted to SASBDB: 2023 Aug 10
Published in SASBDB:

SASDSP8 – RNA Device 43 in ligand-free state (apo-D43)

RNA Device 43 in ligand-free state experimental SAS data
OTHER model
Sample: RNA Device 43 in ligand-free state monomer, 40 kDa synthetic construct RNA
Buffer: 10 mM Bis-Tris, 100 mM KCl, and 1 mM MgCl2, pH: 6.8
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 25
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 58 nm3

SASDSQ8 – RNA Device 43 in ligand-bound state (holo-D43)

RNA Device 43 in ligand-bound state experimental SAS data
OTHER model
Sample: RNA Device 43 in ligand-bound state monomer, 40 kDa synthetic construct RNA
Buffer: 10 mM Bis-Tris, 100 mM KCl, and 1 mM MgCl2, pH: 6.8
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 25
RgGuinier 3.3 nm
Dmax 12.1 nm
VolumePorod 56 nm3