Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state

Ryzhykau Y, Povarova O, Dronova E, Kuklina D, Antifeeva I, Ilyinsky N, Okhrimenko I, Semenov Y, Kuklin A, Ivanovich V, Fonin A, Uversky V, Turoverov K, Kuznetsova I
Biochemical and Biophysical Research Communications :149340 (2023 Nov)

doi: 10.1016/j.bbrc.2023.149340 		Submitted to SASBDB: 2023 Nov 24
Published in SASBDB:

SASDTX4 – G-actin - Skeletal muscle actin from Oryctolagus cuniculus: globular actin monomers

Actin, alpha skeletal muscle experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer: 5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1
RgGuinier 2.9 nm
Dmax 8.5 nm
VolumePorod 58 nm3

SASDTY4 – F-actin - Skeletal muscle actin from Oryctolagus cuniculus: fibrillar actin

Actin, alpha skeletal muscle experimental SAS data
DAMMIF model
Sample: Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer: 5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 1.0 mM ATP, 50 mM KCl, 2 mM MgCl2, pH: 8.1
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1
RgGuinier 15.7 nm
Dmax 60.0 nm
VolumePorod 4710 nm3

SASDTZ4 – I-actin - Skeletal muscle actin from Oryctolagus cuniculus: oligomer of time-inactivated actin (peak SEC fraction)

Actin, alpha skeletal muscle experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer: 5 mM Tris/Tris-HCl, 2.0 mM EDTA, pH: 8.1
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Nov 16
RgGuinier 4.9 nm
Dmax 22.0 nm
VolumePorod 549 nm3

SASDT25 – G- / I-actin mixture - Skeletal muscle actin from Oryctolagus cuniculus: mixture of globular actin monomers and inactivated actin monomers and dimers

Actin, alpha skeletal muscle experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer: 5 mM Tris, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 5
RgGuinier 3.1 nm
Dmax 12.0 nm
VolumePorod 84 nm3