Functional Validation of SAM Riboswitch Element A from Listeria monocytogenes.

Hall I, Zablock K, Sobetski R, Weidmann CA, Keane SC
Biochemistry (2024 Sep 25)

PMID: 39323220 doi: 10.1021/acs.biochem.4c00247

Submitted to SASBDB: 2023 Sep 12 Published in SASBDB: 2024 Jun 27

SASDSK9 – SAM riboswitch element A aptamer domain Sample: SAM riboswitch element A aptamer domain (RNA) monomer, 39 kDa Listeria monocytogenes RNA Buffer: 50 mM potassium phosphate, 135 mM NaCl, 5 mM MgCl2, pH: 6.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 9 RgGuinier 3.2 nm Dmax 11.7 nm VolumePorod 76 nm3

SASDSL9 – SAM riboswitch element A aptamer domain with bound S-adenosyl-L-methionine (SAM) Sample: SAM riboswitch element A aptamer domain (RNA) monomer, 39 kDa Listeria monocytogenes RNA S-adenosyl-L-methionine monomer, 0 kDa Buffer: 50 mM potassium phosphate, 135 mM NaCl, 5 mM MgCl2, pH: 6.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 9 RgGuinier 2.7 nm Dmax 10.2 nm VolumePorod 56 nm3