Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments

Han Q, Veríssimo N, Bryant S, Martin A, Huang Y, Pereira J, Santos-Ebinuma V, Zhai J, Bryant G, Drummond C, Greaves T
Advances in Colloid and Interface Science :103242 (2024 Jun)

doi: 10.1016/j.cis.2024.103242 		Submitted to SASBDB: 2023 Sep 14
Published in SASBDB:

SASDSM9 – Hen egg white lysozyme in 1 mol% ethylammonium nitrate

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 20 nm3

SASDSN9 – Buffer over-subtracted hen egg white lysozyme in 1 mol% ethylammonium nitrate

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 28 nm3

SASDSP9 – Buffer under-substracted hen egg white lysozyme in 1mol% ethylammonium nitrate

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 23 nm3