SARS-CoV-2 N-protein variants: N1-246 and IDL176-246

Guillem Hernandez

Submitted to SASBDB: 2023 Sep 21 Published in SASBDB: 2025 Feb 10

SASDSZ9 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 238 µM Sample: Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29 RgGuinier 3.2 nm Dmax 14.0 nm VolumePorod 19 nm3

SASDT22 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 476 µM Sample: Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29 RgGuinier 3.7 nm Dmax 16.0 nm VolumePorod 33 nm3

SASDT32 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 952 µM Sample: Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29 RgGuinier 4.1 nm Dmax 18.0 nm VolumePorod 53 nm3

SASDT42 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 238 µM Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29 RgGuinier 2.4 nm Dmax 11.0 nm VolumePorod 10 nm3

SASDT52 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 476 µM Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29 RgGuinier 2.7 nm Dmax 11.0 nm VolumePorod 12 nm3

SASDT62 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 952 µM Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29 RgGuinier 2.7 nm Dmax 12.0 nm VolumePorod 13 nm3

SASDSR9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 38.2 µM Sample: Nucleoprotein monomer, 27 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 3.3 nm Dmax 15.5 nm VolumePorod 56 nm3

SASDSS9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 76.4 µM Sample: Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 4.0 nm Dmax 18.0 nm VolumePorod 67 nm3

SASDST9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 294.7 µM Sample: Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 5.2 nm Dmax 22.0 nm VolumePorod 117 nm3

SASDSU9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 400 µM Sample: Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 5.4 nm Dmax 24.0 nm VolumePorod 157 nm3

SASDSV9 – SARS-CoV-2 N-protein (N1-245; residues 1-245) L223P, L227P and L230P triple mutant: 36.4 µM Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29 RgGuinier 3.2 nm Dmax 13.5 nm VolumePorod 53 nm3

SASDSW9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 72.7 µM Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29 RgGuinier 3.5 nm Dmax 14.5 nm VolumePorod 47 nm3

SASDSX9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 294 µM Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29 RgGuinier 4.4 nm Dmax 19.0 nm VolumePorod 68 nm3

SASDSY9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 400 µM Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29 RgGuinier 4.6 nm Dmax 19.0 nm VolumePorod 75 nm3