Design of intrinsically disordered protein variants with diverse structural properties

Pesce F, Bremer A, Tesei G, Hopkins J, Grace C, Mittag T, Lindorff-Larsen K
Science Advances 10(35) (2024 Aug 30)

doi: 10.1126/sciadv.adm9926 		Submitted to SASBDB: 2023 Oct 6
Published in SASBDB:

SASDTK2 – V2 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V2 variant of the low complexity domain of hnRNPA1 experimental SAS data
V2 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V2 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa synthetic construct protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
RgGuinier 2.3 nm
Dmax 8.3 nm
VolumePorod 19 nm3

SASDTL2 – V3 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V3 variant of the low complexity domain of hnRNPA1 experimental SAS data
V3 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V3 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
RgGuinier 2.3 nm
Dmax 9.3 nm
VolumePorod 18 nm3

SASDTM2 – V4 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V4 variant of the low complexity domain of hnRNPA1 experimental SAS data
V4 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V4 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
RgGuinier 2.4 nm
Dmax 9.9 nm
VolumePorod 19 nm3

SASDTN2 – V5 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V5 variant of the low complexity domain of hnRNPA1 experimental SAS data
V5 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V5 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 19 nm3

SASDTJ2 – Low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 experimental SAS data
Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 Kratky plot
Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 monomer, 13 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 19 nm3