Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes.

Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S
PLoS Pathog 20(4):e1012186 (2024 Apr 22)
PMID: 38648216
doi: 10.1371/journal.ppat.1012186 		Submitted to SASBDB: 2023 Nov 28
Published in SASBDB:

SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

Variable surface glycoprotein LiTat 1.5 experimental SAS data
BILBOMD model
Sample: Variable surface glycoprotein LiTat 1.5 dimer, 98 kDa Trypanosoma brucei gambiense protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 170 nm3

SASDTB5 – Trypanosoma brucei gambiense variant surface glycoprotein 3.1

Variant surface glycoprotein 3.1 experimental SAS data
BILBOMD model
Sample: Variant surface glycoprotein 3.1 dimer, 103 kDa Trypanosoma brucei gambiense protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
RgGuinier 4.7 nm
Dmax 17.0 nm
VolumePorod 149 nm3

SASDT75 – Trypanosoma brucei gambiense invariant surface glycoprotein 43 (ISG43)

T. brucei spp.-specific protein experimental SAS data
BILBOMD model
Sample: T. brucei spp.-specific protein monomer, 38 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 53 nm3

SASDT85 – Trypanosoma brucei gambiense invariant surface glycoprotein 64 (ISG64)

64 kDa invariant surface glycoprotein, putative experimental SAS data
BILBOMD model
Sample: 64 kDa invariant surface glycoprotein, putative monomer, 40 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 56 nm3

SASDT95 – Trypanosoma brucei gambiense invariant surface glycoprotein 75 (ISG75)

ISG75 experimental SAS data
BILBOMD model
Sample: ISG75 monomer, 50 kDa Trypanosoma brucei gambiense protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 85 nm3