Alternative splicing controls teneurin-3 compact dimer formation for neuronal recognition

Gogou C, Beugelink J, Frias C, Kresik L, Jaroszynska N, Drescher U, Janssen B, Hindges R, Meijer D
Nature Communications 15(1) (2024 Apr 29)

doi: 10.1038/s41467-024-47763-x

Submitted to SASBDB: 2023 Oct 25 Published in SASBDB: 2024 Apr 30

SASDTY2 – Teneurin-3 A1B1 isoform - 0.97 mg/mL Sample: Isoform A1B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 8.9 nm Dmax 37.0 nm VolumePorod 1059 nm3

SASDT43 – Teneurin-3 A1B1 isoform in 5 mM EDTA - 1.30 mg/mL Sample: Isoform A1B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM EDTA, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 8.8 nm Dmax 35.0 nm VolumePorod 1035 nm3

SASDTZ2 – Teneurin-3 A1B1 isoform in 2 mM calcium - 1.24 mg/mL Sample: Isoform A1B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.9 nm Dmax 33.0 nm VolumePorod 1133 nm3

SASDT23 – Teneurin-3 A1B1 isoform in 2 mM calcium - 0.62 mg/mL Sample: Isoform A1B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.9 nm Dmax 32.0 nm VolumePorod 1129 nm3

SASDT33 – Teneurin-3 A1B1 isoform in 2 mM calcium - 0.31 mg/mL Sample: Isoform A1B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.9 nm Dmax 33.0 nm VolumePorod 1129 nm3

SASDT53 – Teneurin-3 A0B0 isoform - 0.44 mg/mL Sample: Isoform A0B0 of Teneurin-3 dimer, 526 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 9.4 nm Dmax 39.0 nm VolumePorod 1101 nm3

SASDT83 – Teneurin-3 A0B0 isoform in 5 mM EDTA - 0.52 mg/mL Sample: Isoform A0B0 of Teneurin-3 dimer, 526 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM EDTA, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 8.6 nm Dmax 33.0 nm VolumePorod 990 nm3

SASDT63 – Teneurin-3 A0B0 isoform in 2 mM calcium - 0.46 mg/mL Sample: Isoform A0B0 of Teneurin-3 dimer, 526 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.3 nm Dmax 31.0 nm VolumePorod 1057 nm3

SASDT73 – Teneurin-3 A0B0 isoform in 2 mM calcium - 0.23 mg/mL Sample: Isoform A0B0 of Teneurin-3 dimer, 526 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.3 nm Dmax 31.0 nm VolumePorod 1077 nm3

SASDT93 – Teneurin-3 A0B1 isoform - 0.65 mg/mL Sample: Isoform A0B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 9.4 nm Dmax 33.5 nm VolumePorod 1032 nm3

SASDTD3 – Teneurin-3 A0B1 isoform in 5 mM EDTA - 0.62 mg/mL Sample: Isoform A0B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM EDTA, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 8.7 nm Dmax 34.0 nm VolumePorod 987 nm3

SASDTA3 – Teneurin-3 A0B1 isoform in 2 mM calcium - 1.43 mg/mL Sample: Isoform A0B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 8.1 nm Dmax 32.0 nm VolumePorod 1071 nm3

SASDTB3 – Teneurin-3 A0B1 isoform in 2 mM calcium - 0.72 mg/mL Sample: Isoform A0B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 8.0 nm Dmax 32.0 nm VolumePorod 1053 nm3

SASDTC3 – Teneurin-3 A0B1 isoform in 2 mM calcium - 0.36 mg/mL Sample: Isoform A0B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.8 nm Dmax 30.0 nm VolumePorod 1022 nm3

SASDTE3 – Teneurin-3 A1B0 isoform - 0.69 mg/mL Sample: Isoform A1B0 of Teneurin-3 dimer, 541 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 9.0 nm Dmax 34.0 nm VolumePorod 1042 nm3

SASDTJ3 – Teneurin-3 A1B0 isoform in 5 mM EDTA - 0.84 mg/mL Sample: Isoform A1B0 of Teneurin-3 dimer, 541 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM EDTA, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 8.9 nm Dmax 34.0 nm VolumePorod 1049 nm3

SASDTF3 – Teneurin-3 A1B0 isoform in 2 mM calcium - 1.44 mg/mL Sample: Isoform A1B0 of Teneurin-3 dimer, 541 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.8 nm Dmax 33.0 nm VolumePorod 1128 nm3

SASDTG3 – Teneurin-3 A1B0 isoform in 2 mM calcium - 0.74 mg/mL Sample: Isoform A1B0 of Teneurin-3 dimer, 541 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.7 nm Dmax 30.0 nm VolumePorod 1089 nm3

SASDTH3 – Teneurin-3 A1B0 isoform in 2 mM calcium - 0.36 mg/mL Sample: Isoform A1B0 of Teneurin-3 dimer, 541 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8 Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10 RgGuinier 7.7 nm Dmax 32.0 nm VolumePorod 1092 nm3