The SCR-17 and SCR-18 glycans in human complement Factor H enhance its regulatory function.

Gao X, Iqbal H, Yu DQ, Gor J, Coker AR, Perkins SJ
J Biol Chem :107624 (2024 Aug 2)
PMID: 39098532
doi: 10.1016/j.jbc.2024.107624 		Submitted to SASBDB: 2024 Feb 12
Published in SASBDB:

SASDUT2 – Recombinant short complement regulator SCR-17/18H (produced in Pichia pastoris with PNGase F treatment)

Complement factor H experimental SAS data
PYMOL model
Sample: Complement factor H monomer, 17 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 137 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 20
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 46 nm3

SASDUU2 – Recombinant short complement regulator SCR-17/18, without glycan (produced in Escherichia coli)

Complement factor H experimental SAS data
PYMOL model
Sample: Complement factor H monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 137 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 20
RgGuinier 2.3 nm
Dmax 7.0 nm
VolumePorod 19 nm3