SARS-CoV-2 N-protein (N1-365, residues 1-365) oligomerization seen by SAXS

Guillem Hernandez

Submitted to SASBDB: 2023 Dec 5 Published in SASBDB: 2025 Feb 10

SASDTE5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 26 µM Sample: Nucleoprotein dimer, 82 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 5.7 nm Dmax 23.0 nm VolumePorod 160 nm3

SASDTF5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 49 µM Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 5.8 nm Dmax 24.0 nm VolumePorod 181 nm3

SASDTG5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 103 µM Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 6.2 nm Dmax 25.0 nm VolumePorod 215 nm3

SASDTH5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 206 µM Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 6.8 nm Dmax 28.0 nm VolumePorod 578 nm3

SASDTJ5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 299 µM Sample: Nucleoprotein hexamer, 245 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 7.1 nm Dmax 29.0 nm VolumePorod 708 nm3