SARS-CoV-2 N-protein Full Length (N1-419, residues 1-419) oligomerization seen by SAXS

Guillem Hernandez

Submitted to SASBDB: 2023 Dec 6 Published in SASBDB: 2025 Feb 10

SASDTX5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 19 µM Sample: Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 6.3 nm Dmax 26.0 nm VolumePorod 233 nm3

SASDTY5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 50 µM Sample: Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 6.9 nm Dmax 28.5 nm VolumePorod 571 nm3

SASDTZ5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 103 µM Sample: Nucleoprotein hexamer, 281 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 7.6 nm Dmax 32.0 nm VolumePorod 764 nm3

SASDT26 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 216 µM Sample: Nucleoprotein octamer, 375 kDa Severe acute respiratory … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13 RgGuinier 8.2 nm Dmax 33.0 nm VolumePorod 1028 nm3