The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry

Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
Nature Communications 15(1) (2024 Aug 24)

doi: 10.1038/s41467-024-51681-3 		Submitted to SASBDB: 2023 Nov 24
Published in SASBDB:

SASDTQ5 – SemDΔAPH (apo form)

Uncharacterized protein experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 67 nm3

SASDTR5 – BR-GBD (apo form of actin nucleation-promoting factor WASL)

Actin nucleation-promoting factor WASL experimental SAS data
Actin nucleation-promoting factor WASL Kratky plot
Sample: Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
RgGuinier 3.0 nm
Dmax 9.9 nm
VolumePorod 19 nm3

SASDTS5 – Sorting nexin-9, N-terminus - SNX9-SH3 (apo form)

Sorting nexin-9 experimental SAS data
Sorting nexin-9 Kratky plot
Sample: Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 42 nm3

SASDTT5 – SemDΔAPH bound to Sorting nexin-9, N-terminus - SNX9-SH3

Uncharacterized proteinSorting nexin-9 experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 94 nm3

SASDTU5 – SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL)

Uncharacterized proteinActin nucleation-promoting factor WASL experimental SAS data
SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL) Rg histogram
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
RgGuinier 4.2 nm
Dmax 15.9 nm
VolumePorod 102 nm3