Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA.

Arentson BW, Hayes EL, Zhu W, Singh H, Tanner JJ, Becker DF
Biosci Rep 36(6) (2016 Dec)

PMID: 27742866 doi: 10.1042/BSR20160435

Submitted to SASBDB: 2016 Oct 10 Published in SASBDB: 2016 Oct 16

SASDB27 – Chimeric EcRHH-RcPutA: The E.coli Proline utilization A RHH domain fused to R.capsulatus PutA Sample: Proline utilization A dimer, 251 kDa Escherchia coli, Rhodobacter … protein Buffer: 50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Oct 16 RgGuinier 5.2 nm Dmax 18.3 nm VolumePorod 308 nm3