Microsecond dynamics control the HIV-1 Envelope conformation.

Bennett AL, Edwards R, Kosheleva I, Saunders C, Bililign Y, Williams A, Bubphamala P, Manosouri K, Anasti K, Saunders KO, Alam SM, Haynes BF, Acharya P, Henderson R
Sci Adv 10(5):eadj0396 (2024 Feb 2)

PMID: 38306419 doi: 10.1126/sciadv.adj0396

Submitted to SASBDB: 2024 Jan 10 Published in SASBDB: 2024 Feb 3

SASDTZ9 – HIV-1 Envelope Glycoprotein SOSIP from a CH505 isolate 53 weeks post-infection at 25 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1 RgGuinier 5.4 nm Dmax 16.7 nm VolumePorod 768 nm3

SASDU22 – HIV-1 Envelope Glycoprotein SOSIP from a CH505 isolate 78 weeks post-infection at 25 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1 RgGuinier 5.1 nm Dmax 16.0 nm VolumePorod 709 nm3

SASDU32 – HIV-1 Envelope Glycoprotein SOSIP from a CH505 isolate 100 weeks post-infection at 25 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1 RgGuinier 5.2 nm Dmax 17.2 nm VolumePorod 770 nm3

SASDU42 – HIV-1 Envelope Glycoprotein SOSIP from a BG505 isolate containing T332N mutation at 25 °C Sample: BG505SOSIP.664T332N Env glycoprotein trimer, 213 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1 RgGuinier 5.0 nm Dmax 20.7 nm VolumePorod 712 nm3

SASDT29 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 25 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27 RgGuinier 4.6 nm Dmax 15.5 nm VolumePorod 705 nm3

SASDT39 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 35 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27 RgGuinier 4.6 nm Dmax 15.4 nm VolumePorod 704 nm3

SASDT49 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 44 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27 RgGuinier 4.6 nm Dmax 15.6 nm VolumePorod 702 nm3

SASDT59 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 50 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29 RgGuinier 4.6 nm Dmax 15.6 nm VolumePorod 699 nm3

SASDT69 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1.5 µs post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30 RgGuinier 4.6 nm Dmax 15.5 nm VolumePorod 706 nm3

SASDT79 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 3 µs post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30 RgGuinier 4.6 nm Dmax 15.5 nm VolumePorod 706 nm3

SASDT89 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 5 µs post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30 RgGuinier 4.6 nm Dmax 15.5 nm VolumePorod 706 nm3

SASDT99 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 10 µs post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28 RgGuinier 4.3 nm Dmax 15.3 nm VolumePorod 729 nm3

SASDTA9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 50 µs post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28 RgGuinier 4.3 nm Dmax 15.4 nm VolumePorod 729 nm3

SASDTB9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 100 µs post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28 RgGuinier 4.3 nm Dmax 15.3 nm VolumePorod 730 nm3

SASDTC9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 500 µs post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28 RgGuinier 4.3 nm Dmax 15.3 nm VolumePorod 728 nm3

SASDTD9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1 ms (20 Hz) post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28 RgGuinier 4.3 nm Dmax 15.3 nm VolumePorod 742 nm3

SASDTE9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1 ms (5 Hz) post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29 RgGuinier 4.1 nm Dmax 15.4 nm VolumePorod 706 nm3

SASDTF9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 10 ms post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29 RgGuinier 4.1 nm Dmax 15.4 nm VolumePorod 706 nm3

SASDTG9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 100 ms post temperature jump (44 °C - 50 °C) Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29 RgGuinier 4.1 nm Dmax 15.5 nm VolumePorod 706 nm3

SASDTH9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 30 °C Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28 RgGuinier 4.1 nm Dmax 15.3 nm VolumePorod 768 nm3

SASDTJ9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 35 °C Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28 RgGuinier 5.3 nm Dmax 15.3 nm VolumePorod 765 nm3

SASDTK9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 40 °C Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28 RgGuinier 5.3 nm Dmax 15.3 nm VolumePorod 764 nm3

SASDTL9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 44 °C Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28 RgGuinier 5.2 nm Dmax 15.2 nm VolumePorod 728 nm3

SASDTM9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 5 µs post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.1 nm VolumePorod 750 nm3

SASDTN9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 10 µs post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.1 nm VolumePorod 750 nm3

SASDTP9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 50 µs post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.1 nm VolumePorod 752 nm3

SASDTQ9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 100 µs post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.1 nm VolumePorod 755 nm3

SASDTR9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 250 µs post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.3 nm VolumePorod 741 nm3

SASDTS9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 500 µs post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.1 nm VolumePorod 750 nm3

SASDTT9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 750 µs post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.3 nm VolumePorod 739 nm3

SASDTU9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 1 ms post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.1 nm VolumePorod 751 nm3

SASDTV9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 10 ms post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.5 nm VolumePorod 733 nm3

SASDTW9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 100 ms post temperature jump (44 °C - 50 °C) Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30 RgGuinier 5.3 nm Dmax 15.3 nm VolumePorod 732 nm3

SASDTX9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate including the 4.1 stabilization mutations at 25 °C Sample: CH505TF.6R.SOSIP.664.v4.1 Env glycoprotein trimer, 218 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1 RgGuinier 5.1 nm Dmax 18.2 nm VolumePorod 718 nm3

SASDTY9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 25 °C Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1 RgGuinier 5.3 nm Dmax 16.1 nm VolumePorod 731 nm3