A secreted bacterial protein protects bacteria from cationic antimicrobial peptides by entrapment in phase separated droplets

Ostan N, Cole G, Wang F, Reichheld S, Moore G, Pan C, Yu R, Lai C, Sharpe S, Lee H, Schryvers A, Moraes T
PNAS Nexus (2024 Apr 02)

doi: 10.1093/pnasnexus/pgae139

Submitted to SASBDB: 2023 Dec 19 Published in SASBDB: 2024 Apr 5

SASDTS7 – Bovine Lactoferrin Sample: Lactoferrin monomer, 75 kDa Bos taurus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 23 RgGuinier 3.2 nm Dmax 11.5 nm VolumePorod 117 nm3

SASDTT7 – Lactoferrin binding protein B Sample: Transferrin-binding protein B monomer, 97 kDa Moraxella bovis protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 23 RgGuinier 3.7 nm Dmax 12.4 nm VolumePorod 130 nm3

SASDTU7 – Lactoferrin binding protein B (C-terminal alpha helical domain) Sample: Transferrin-binding protein B monomer, 32 kDa Moraxella bovis protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 23 RgGuinier 3.2 nm Dmax 11.9 nm VolumePorod 49 nm3

SASDTV7 – Lactoferrin binding protein B (C-terminal alpha helical domain) bound to bovine lactoferrin Sample: Lactoferrin monomer, 75 kDa Bos taurus protein Transferrin-binding protein B monomer, 32 kDa Moraxella bovis protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 23 RgGuinier 5.6 nm Dmax 20.3 nm VolumePorod 318 nm3