Thermodynamic coupling of the tandem RRM domains of hnRNP A1 underlie its pleiotropic RNA binding functions.

Levengood JD, Potoyan D, Penumutchu S, Kumar A, Zhou Q, Wang Y, Hansen AL, Kutluay S, Roche J, Tolbert BS
Sci Adv 10(28):eadk6580 (2024 Jul 12)

PMID: 38985864 doi: 10.1126/sciadv.adk6580

Submitted to SASBDB: 2023 Dec 20 Published in SASBDB: 2024 Jan 31

SASDTW7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A; SEC-SAXS, July 2022) Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 7 RgGuinier 2.9 nm Dmax 12.0 nm VolumePorod 54 nm3

SASDTX7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A; SEC-SAXS, Sept 2022) Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Sep 27 RgGuinier 2.9 nm Dmax 12.0 nm VolumePorod 53 nm3

SASDTY7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 0.1 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.9 nm

SASDTZ7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 50 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.0 nm

SASDT28 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 100 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.0 nm

SASDT38 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 150 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.3 nm

SASDT48 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 200 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.2 nm

SASDT58 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 250 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.3 nm

SASDT68 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 0.1 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.6 nm Dmax 13.0 nm VolumePorod 36 nm3

SASDT78 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 50 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.6 nm Dmax 11.0 nm VolumePorod 33 nm3

SASDT88 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 100 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.5 nm Dmax 10.0 nm VolumePorod 32 nm3

SASDT98 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 150 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.4 nm Dmax 10.0 nm VolumePorod 29 nm3

SASDTA8 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 200 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.4 nm Dmax 9.5 nm VolumePorod 28 nm3

SASDTB8 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 250 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.4 nm Dmax 9.5 nm VolumePorod 29 nm3

SASDTC8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut); SEC-SAXS, July 2022) Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 7 RgGuinier 3.4 nm Dmax 14.0 nm VolumePorod 57 nm3

SASDTD8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut); SEC-SAXS, Sept 2022) Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Sep 27 RgGuinier 3.7 nm Dmax 14.0 nm VolumePorod 58 nm3

SASDTE8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 0.1 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.6 nm Dmax 20.0 nm VolumePorod 98 nm3

SASDTF8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 50 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.7 nm Dmax 23.0 nm VolumePorod 104 nm3

SASDTG8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 100 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.6 nm Dmax 23.0 nm VolumePorod 94 nm3

SASDTH8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 150 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.6 nm Dmax 23.0 nm VolumePorod 109 nm3

SASDTJ8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 200 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.7 nm

SASDTK8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 250 MPa Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.6 nm

SASDTL8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 0.1 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.8 nm

SASDTM8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 50 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.0 nm

SASDTN8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 100 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.2 nm

SASDTP8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 150 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 2.9 nm

SASDTQ8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 200 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.1 nm

SASDTR8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 250 MPa Sample: Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein Buffer: 100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1 RgGuinier 3.2 nm