Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5

Tomas Klumpler

Submitted to SASBDB: 2023 Dec 21 Published in SASBDB: 2025 Feb 10

SASDTK7 – RECQ like helicase 5 (1 mg/ml) Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Mar 29 RgGuinier 6.5 nm Dmax 19.5 nm VolumePorod 310 nm3

SASDTL7 – RECQ like helicase 5 (4 mg/ml) Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Mar 29 RgGuinier 6.4 nm Dmax 20.2 nm VolumePorod 288 nm3

SASDTM7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.2 mg/ml) Sample: ATP-dependent DNA helicase Q5, 21 kDa Homo sapiens protein Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19 RgGuinier 3.7 nm Dmax 9.3 nm VolumePorod 73 nm3

SASDTN7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.4 mg/ml) Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19 RgGuinier 3.9 nm Dmax 10.1 nm VolumePorod 91 nm3

SASDTP7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.6 mg/ml) Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19 RgGuinier 4.3 nm Dmax 11.2 nm VolumePorod 116 nm3

SASDTQ7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.8 mg/ml) Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19 RgGuinier 4.6 nm Dmax 13.2 nm VolumePorod 102 nm3

SASDTR7 – RECQ like helicase 5 fragment (amino acids 625-825; 1.0 mg/ml) Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19 RgGuinier 5.7 nm Dmax 16.2 nm VolumePorod 150 nm3