Structural basis for recruitment of peptidoglycan endopeptidase MepS by lipoprotein NlpI.

Wang S, Huang CH, Lin TS, Yeh YQ, Fan YS, Wang SW, Tseng HC, Huang SJ, Chang YY, Jeng US, Chang CI, Tzeng SR
Nat Commun 15(1):5461 (2024 Jun 27)
PMID: 38937433
doi: 10.1038/s41467-024-49552-y 		Submitted to SASBDB: 2024 Jun 29
Published in SASBDB:

SASDVW3 – Lipoprotein NlpI dimer bound to two asymmetrical murein DD-endopeptidase mMepS dimers at pH 7.0

Lipoprotein NlpIMurein DD-endopeptidase MepS/Murein LD-carboxypeptidase experimental SAS data
PYMOL model
Sample: Lipoprotein NlpI dimer, 63 kDa Escherichia coli (strain … protein
Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase tetramer, 73 kDa Escherichia coli (strain … protein
Buffer: 0.2 M Na/K phosphate pH 7.0, 2 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at TPS13A, NSRRC on 2023 Sep 7
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 123 nm3