Fusion then fission: splitting and reassembly of an artificial fusion-protein nanocage.

Ohara N, Kawakami N, Arai R, Adachi N, Ikeda A, Senda T, Miyamoto K
Chem Commun (Camb) (2024 Apr 8)
PMID: 38586927
doi: 10.1039/d4cc00115j 		Submitted to SASBDB: 2024 Jan 23
Published in SASBDB:

SASDUC2 – TIP120 (designed based on Truncated Icosahedral Protein composed of 60-mer fusion proteins)

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsC-terminal split fragment of a single subunit of Truncated Isosahedral Protein composed of 60-mer fusion proteins experimental SAS data
DAMMIN model
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins, 360 kDa synthetic construct protein
C-terminal split fragment of a single subunit of Truncated Isosahedral Protein composed of 60-mer fusion proteins, 715 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
RgGuinier 9.2 nm
Dmax 22.5 nm
VolumePorod 3480 nm3

SASDUD2 – mTIP120-Ba (metal-ion induced 120-mer complex of TIP120 mutant (K26E in C-terminal subunit) with Ba ions)

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsK26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins experimental SAS data
DAMMIN model
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins, 360 kDa synthetic construct protein
K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins, 857 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 4 mM BaCl2, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
RgGuinier 9.4 nm
Dmax 22.4 nm
VolumePorod 3320 nm3

SASDUE2 – TIP120 mutant K26E in CoreC (C-terminal split fragment of a fusion protein that assembles to Truncated Icosahedral protein composed of TIP60 ) subunit with EDTA

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsK26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins experimental SAS data
N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins Kratky plot
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins, 360 kDa synthetic construct protein
K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins, 857 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 4 mM BaCl2, 32 mM EDTA, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
RgGuinier 4.7 nm
Dmax 13.9 nm
VolumePorod 110 nm3