A dynamic structural unit of phase-separated heterochromatin protein 1α as revealed by integrative structural analyses

Furukawa A, Yonezawa K, Negami T, Yoshimura Y, Hayashi A, Nakayama J, Adachi N, Senda T, Shimizu K, Terada T, Shimizu N, Nishimura Y
Nucleic Acids Research 53(6) (2025 Mar 24)

doi: 10.1093/nar/gkaf154

Submitted to SASBDB: 2024 Feb 16 Published in SASBDB: 2025 Mar 25

SASDU23 – Heterochromatin protein HP1α, S97A mutant, full-length (HP1α_S97A) in 50 mM NaCl Sample: Heterochromatin protein HP1α, S97A mutant, full-length dimer, 41 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 50 mM NaCl, 1 mM DTT, pH: 7 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 Jun 2 RgGuinier 4.0 nm Dmax 14.4 nm VolumePorod 117 nm3

SASDU33 – Phosphorylated heterochromatin protein HP1α, S97A mutant, full-length (pHP1α_S97A) in 50 mM NaCl Sample: Phosphorylated Heterochromatin protein HP1α, S97A mutant, full-length dimer, 41 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 50 mM NaCl, 1 mM DTT, pH: 7 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 Jun 2 RgGuinier 3.8 nm Dmax 19.5 nm VolumePorod 129 nm3

SASDU43 – Heterochromatin protein HP1α, S97A mutant, full-length (HP1α_S97A) in 500 mM NaCl Sample: Heterochromatin protein HP1α, S97A mutant, full-length dimer, 41 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 500 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 Nov 19 RgGuinier 4.5 nm Dmax 17.1 nm VolumePorod 94 nm3

SASDU53 – Phosphorylated heterochromatin protein HP1α, S97A mutant, full-length (pHP1α_S97A) in 500 mM NaCl Sample: Phosphorylated Heterochromatin protein HP1α, S97A mutant, full-length dimer, 41 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 500 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 Nov 19 RgGuinier 4.6 nm Dmax 17.7 nm VolumePorod 104 nm3

SASDU63 – Heterochromatin protein HP1α, delta CSD mutant, in 50 mM NaCl Sample: Heterochromatin protein HP1α, delta CSD mutant monomer, 13 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 50 mM NaCl, 1 mM DTT, pH: 7 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 27 RgGuinier 2.8 nm Dmax 13.0 nm VolumePorod 23 nm3

SASDU73 – Phosphorylated heterochromatin protein HP1α, delta CSD mutant, in 50 mM NaCl Sample: Phosphorylated Heterochromatin protein HP1α, delta CSD mutant monomer, 13 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 50 mM NaCl, 1 mM DTT, pH: 7 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 27 RgGuinier 3.0 nm Dmax 14.4 nm VolumePorod 44 nm3

SASDU83 – Phosphorylated heterochromatin protein HP1α, delta CSD b4 mutant, in 50 mM NaCl Sample: Phosphorylated Heterochromatin protein HP1α, delta CSD b4 mutant monomer, 13 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 50 mM NaCl, 1 mM DTT, pH: 7 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 27 RgGuinier 2.7 nm Dmax 13.0 nm VolumePorod 34 nm3