A glimpse into the hidden world of the flexible C-terminal protein binding domains of human RAD52

Struble L, Lovelace J, Borgstahl G
Journal of Structural Biology 216(3):108115 (2024 Sep)

doi: 10.1016/j.jsb.2024.108115 		Submitted to SASBDB: 2024 Aug 7
Published in SASBDB:

SASDVZ5 – Human DNA repair protein RAD52 homolog (amino acids 1-212)

DNA repair protein RAD52 homolog experimental SAS data
DAMMIF model
Sample: DNA repair protein RAD52 homolog decamer, 234 kDa Homo sapiens protein
Buffer: 20 mM Bis-Tris, 10% glycerol, 400 mM NaCl, 100 mM KCl, 1 mM EDTA, pH: 6
Experiment: SAXS data collected at Rigaku BioSAXS 1000, Eppley Structural Biology Facility, University of Nebraska Medical Center on 2014 Jul 25
RgGuinier 4.1 nm
Dmax 11.8 nm
VolumePorod 375 nm3

SASDV26 – Human DNA repair protein RAD52 homolog (amino acids 1-303)

DNA repair protein RAD52 homolog experimental SAS data
DAMMIF model
Sample: DNA repair protein RAD52 homolog nonamer, 300 kDa Homo sapiens protein
Buffer: 20 mM Bis-Tris, 10% GLycerol, 400 mM NaCl, 100 mM KCl, 1mM EDTA, pH: 6
Experiment: SAXS data collected at Rigaku BioSAXS 1000, Eppley Structural Biology Facility, University of Nebraska Medical Center on 2014 Jul 25
RgGuinier 4.8 nm
Dmax 18.3 nm
VolumePorod 782 nm3