The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction.

Kyriukha Y, Watkins MB, Redington JM, Chintalapati N, Ganti A, Dastvan R, Uversky VN, Hopkins JB, Pozzi N, Korolev S
iScience 27(12):111259 (2024 Dec 20)

PMID: 39584160 doi: 10.1016/j.isci.2024.111259

Submitted to SASBDB: 2024 Oct 5 Published in SASBDB: 2024 Dec 23

SASDSZ5 – DNA binding domain of PALB2 (partner and localizer of BRCA2) in 500 mM NaCl Sample: Partner and localizer of BRCA2 dimer, 46 kDa Homo sapiens protein Buffer: 50 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 11 RgGuinier 5.5 nm Dmax 25.4 nm VolumePorod 171 nm3

SASDS26 – DNA binding domain of PALB2 (partner and localizer of BRCA2) in 160 mM NaCl Sample: Partner and localizer of BRCA2 dimer, 46 kDa Homo sapiens protein Buffer: 50 mM HEPES, 160 mM NaCl, 0.5 mM TCEP, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 11 RgGuinier 4.7 nm Dmax 21.3 nm VolumePorod 197 nm3

SASDVQ7 – PABL2 (Partner and localizer of BRCA2) L24A mutant Sample: Partner and localizer of BRCA2 (L24A) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 160 mM NaCl, 0.5 mM TCEP pH 7.5, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Dec 9 RgGuinier 5.2 nm Dmax 21.6 nm VolumePorod 127 nm3

SASDVR7 – PABL2 (Partner and localizer of BRCA2) incubated with dT50 (DNA) Sample: Partner and localizer of BRCA2 dimer, 46 kDa Homo sapiens protein Buffer: 20 mM HEPES, 160 mM NaCl, 0.5 mM TCEP pH 7.5, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Dec 9 RgGuinier 4.6 nm Dmax 17.6 nm VolumePorod 236 nm3