A General Mechanism for Initiating the General Stress Response in Bacteria.

Baral R, Ho K, Kumar RP, Hopkins JB, Watkins MB, LaRussa S, Caban-Penix S, Calderone LA, Bradshaw N
bioRxiv (2025 Jan 22)
PMID: 38405867
doi: 10.1101/2024.02.16.580724 		Submitted to SASBDB: 2024 Mar 21
Published in SASBDB:

SASDU85 – Wild-type phosphoserine phosphatase RsbU dimer

Phosphoserine phosphatase RsbU experimental SAS data
MULTIFOXS model
Sample: Phosphoserine phosphatase RsbU dimer, 77 kDa Bacillus subtilis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 24
RgGuinier 3.9 nm
Dmax 14.3 nm
VolumePorod 117 nm3

SASDU95 – Hyperactive variant of phosphoserine phosphatase RsbU (Q94L) bound to the activator serine/threonine-protein kinase RsbT (heterotetrameric complex)

Serine/threonine-protein kinase RsbTPhosphoserine phosphatase RsbU (Q94L) experimental SAS data
Serine/threonine-protein kinase RsbT Phosphoserine phosphatase RsbU (Q94L) Kratky plot
Sample: Serine/threonine-protein kinase RsbT dimer, 29 kDa Bacillus subtilis (strain … protein
Phosphoserine phosphatase RsbU (Q94L) dimer, 77 kDa Bacillus subtilis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 24
RgGuinier 3.5 nm
Dmax 12.3 nm
VolumePorod 119 nm3