The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration.

Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
Proc Natl Acad Sci U S A 121(13):e2319998121 (2024 Mar 26)

PMID: 38513096 doi: 10.1073/pnas.2319998121

Submitted to SASBDB: 2024 Feb 19 Published in SASBDB: 2024 Mar 4

SASDUG3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer Sample: Endoglucanase-7 monomer, 30 kDa Colletotrichum orbiculare (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1 RgGuinier 3.0 nm Dmax 12.0 nm VolumePorod 50 nm3

SASDUH3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer Sample: Endoglucanase-7 dimer, 60 kDa Colletotrichum orbiculare (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1 RgGuinier 4.8 nm Dmax 21.7 nm VolumePorod 83 nm3

SASDUJ3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) deltaX283 Sample: Endoglucanase-7 monomer, 27 kDa Colletotrichum orbiculare (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1 RgGuinier 1.8 nm Dmax 6.3 nm VolumePorod 32 nm3

SASDUK3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) C286A* Sample: Endoglucanase-7 (C286A) monomer, 30 kDa Colletotrichum orbiculare (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1 RgGuinier 3.0 nm Dmax 11.6 nm VolumePorod 48 nm3

SASDUL3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer Sample: Glycosyl hydrolase family 61 monomer, 30 kDa Colletotrichum higginsianum (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9 RgGuinier 4.1 nm Dmax 14.6 nm VolumePorod 85 nm3

SASDUM3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer Sample: Glycosyl hydrolase family 61 dimer, 61 kDa Colletotrichum higginsianum (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9 RgGuinier 6.2 nm Dmax 24.5 nm VolumePorod 206 nm3

SASDUN3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) monomer Sample: Endoglucanase-4 monomer, 33 kDa Colletotrichum higginsianum (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17 RgGuinier 3.9 nm Dmax 17.0 nm VolumePorod 68 nm3

SASDUP3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) dimer Sample: Endoglucanase-4 dimer, 66 kDa Colletotrichum higginsianum (strain … protein Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17 RgGuinier 6.4 nm Dmax 27.9 nm VolumePorod 199 nm3