Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes.

Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
Int J Biol Macromol :134556 (2024 Aug 9)

PMID: 39128762 doi: 10.1016/j.ijbiomac.2024.134556

Submitted to SASBDB: 2024 Feb 28 Published in SASBDB: 2024 Mar 8

SASDUW3 – Calcium activated full-length gelsolin Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Buffer: 25 mM Tris-HCl, pH8, 45 mM NaCl, 1 mM EGTA + 4 mM CaCl2, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 4.2 nm Dmax 16.0 nm VolumePorod 129 nm3

SASDUX3 – F-actin in F-buffer at an actin concentration of 2 mg/mL Sample: Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 13.4 nm Dmax 48.0 nm

SASDUY3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:32 molar ratio in F-actin buffer Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 12.2 nm

SASDUZ3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:16 molar ratio in F-actin buffer Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 14.5 nm

SASDU24 – Calcium activated full-length gelsolin and the F-form of actin at a 1:8 molar ratio in F-actin buffer Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 13.6 nm

SASDU34 – Calcium activated full-length gelsolin and the F-form of actin at a 1:4 molar ratio in F-actin buffer Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 8.8 nm

SASDU44 – Calcium activated full-length gelsolin and the F-form of actin at a 1:2 molar ratio in F-actin buffer Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 7.3 nm

SASDU54 – Calcium activated full-length gelsolin and the F-form of actin at a 1:1 molar ratio in F-actin buffer Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 5.2 nm

SASDU64 – Calcium-gelsolin and the F-form of actin at a 1:2 molar ratio from high to low ionic strength Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 4.7 nm Dmax 25.0 nm VolumePorod 262 nm3

SASDU74 – Calcium-gelsolin and the F-form of actin at a 1:1 molar ratio from high to low ionic strength Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 5.2 nm Dmax 25.0 nm VolumePorod 266 nm3

SASDU84 – Calcium-gelsolin and the G-form of actin at a 1:2 molar ratio in low ionic strength (SEC-SAXS) Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31 RgGuinier 4.4 nm Dmax 25.0 nm VolumePorod 241 nm3

SASDU94 – Calcium-gelsolin and the G-form of actin at a 1:1 molar ratio in low ionic strength (SEC-SAXS) Sample: Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 1 RgGuinier 4.6 nm Dmax 25.0 nm VolumePorod 243 nm3