Structural analysis reveals how tetrameric tyrosine-phosphorylated STAT1 is targeted by the rabies virus P-protein

Sugiyama A, Minami M, Ugajin K, Inaba-Inoue S, Yabuno N, Takekawa Y, Xiaomei S, Takei S, Sasaki M, Nomai T, Jiang X, Kita S, Maenaka K, Hirose M, Yao M, Gooley P, Moseley G, Sugita Y, Ose T
Science Signaling 18(878) (2025 Mar 18)

doi: 10.1126/scisignal.ads2210

Submitted to SASBDB: 2025 Mar 25 Published in SASBDB: 2025 Mar 25

SASDUL4 – Signal transducer and activator of transcription 1-alpha/beta (STAT1) Sample: Signal transducer and activator of transcription 1-alpha/beta tetramer, 349 kDa Homo sapiens protein Buffer: 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH: 7.4 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Dec 11 RgGuinier 6.2 nm Dmax 21.2 nm VolumePorod 800 nm3

SASDUM4 – Signal transducer and activator of transcription 1-alpha/beta bound to phosphoprotein (STAT1/RVP) Sample: Signal transducer and activator of transcription 1-alpha/beta tetramer, 349 kDa Homo sapiens protein Phosphoprotein monomer, 33 kDa Rabies virus (strain … protein Buffer: 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH: 7.4 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Dec 11 RgGuinier 6.5 nm Dmax 26.0 nm VolumePorod 857 nm3

SASDUN4 – Signal transducer and activator of transcription 1-alpha/beta bound to transducer/activator DNA (STAT1/DNA) Sample: Signal transducer and activator of transcription 1-alpha/beta tetramer, 349 kDa Homo sapiens protein Signal transducer and activator of transcription 1 binding DNA dimer, 11 kDa Homo sapiens DNA Buffer: 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH: 7.4 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Dec 11 RgGuinier 6.1 nm Dmax 19.4 nm VolumePorod 761 nm3