The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures

Bradshaw W, Harris G, Gileadi O, Katis V
Structure (2024 Oct)

doi: 10.1016/j.str.2024.09.024

Submitted to SASBDB: 2024 Apr 29 Published in SASBDB: 2024 Oct 7

SASDUT7 – The tandem SH2 domains of Tyrosine-protein kinase SYK Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13 RgGuinier 2.2 nm Dmax 6.9 nm VolumePorod 49 nm3

SASDUU7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound FCER1G diphospho-ITAM peptide Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein High affinity immunoglobulin epsilon receptor subunit gamma monomer, 2 kDa Homo sapiens protein Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13 RgGuinier 2.1 nm Dmax 7.0 nm VolumePorod 54 nm3

SASDUV7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound CD3G diphospho-ITAM peptide Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein T-cell surface glycoprotein CD3 gamma chain monomer, 3 kDa Homo sapiens protein Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13 RgGuinier 2.1 nm Dmax 7.1 nm VolumePorod 56 nm3

SASDUW7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound TYROBP diphospho-ITAM peptide Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein TYRO protein tyrosine kinase-binding protein monomer, 2 kDa Homo sapiens protein Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13 RgGuinier 2.1 nm Dmax 7.1 nm VolumePorod 53 nm3