Phase separation of a microtubule plus-end tracking protein into a fluid fractal network

Czub M, Uliana F, Grubić T, Padeste C, Rosowski K, Dufresne E, Menzel A, Vakonakis I, Gasser U, Steinmetz M
(2024 Apr 19)

doi: 10.1101/2024.04.19.590270

Submitted to SASBDB: 2024 Apr 6 Published in SASBDB: 2024 Jul 15

SASDUT6 – Nuclear fusion protein BIK1 in high salt buffer Sample: Nuclear fusion protein BIK1 dimer, 106 kDa Saccharomyces cerevisiae (strain … protein Buffer: 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jul 13 RgGuinier 8.8 nm Dmax 28.0 nm VolumePorod 467 nm3

SASDUU6 – Nuclear fusion protein BIK1 coiled-coil in a high salt buffer Sample: Nuclear fusion protein BIK1 dimer, 55 kDa Saccharomyces cerevisiae (strain … protein Buffer: 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jul 13 RgGuinier 8.0 nm Dmax 27.0 nm VolumePorod 282 nm3

SASDUV6 – Nuclear fusion protein BIK1 ΔQQFF in a high salt buffer Sample: Nuclear fusion protein BIK1 dimer, 105 kDa Saccharomyces cerevisiae (strain … protein Buffer: 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jul 13 RgGuinier 9.4 nm Dmax 29.4 nm VolumePorod 632 nm3