The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans.

Rehman S, Antonovic AK, McIntire IE, Zheng H, Cleaver L, Baczynska M, Adams CO, Portlock T, Richardson K, Shaw R, Oregioni A, Mastroianni G, Whittaker SB, Kelly G, Lorenz CD, Fornili A, Cianciotto NP, Garnett JA
Nat Commun 15(1):4912 (2024 Jun 8)

PMID: 38851738 doi: 10.1038/s41467-024-49255-4

Submitted to SASBDB: 2024 Apr 14 Published in SASBDB: 2024 Jun 11

SASDUG7 – Wild type Lcl C-terminal domain Sample: HbP1 trimer, 56 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.8 nm Dmax 9.7 nm VolumePorod 105 nm3

SASDUH7 – Lcl C-terminal domain R477A mutant - trimer Sample: HbP1 (R477A) trimer, 55 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.7 nm Dmax 9.4 nm VolumePorod 79 nm3

SASDUJ7 – Lcl C-terminal domain R477A mutant - monomer Sample: HbP1 (R477A) monomer, 18 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 1.9 nm Dmax 6.4 nm VolumePorod 33 nm3

SASDUK7 – Lcl C-terminal domain E503A mutant Sample: HbP1 (E503A) trimer, 56 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.8 nm Dmax 9.9 nm VolumePorod 106 nm3

SASDUL7 – Lcl C-terminal domain K504A mutant Sample: HbP1 (K504A) trimer, 56 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.8 nm Dmax 9.7 nm VolumePorod 106 nm3

SASDUM7 – Lcl C-terminal domain K515A mutant Sample: HbP1 (K515A) trimer, 56 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.8 nm Dmax 9.8 nm VolumePorod 105 nm3

SASDUN7 – Lcl C-terminal domain K520A mutant Sample: HbP1 (K520A) trimer, 56 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.8 nm Dmax 9.8 nm VolumePorod 100 nm3

SASDUP7 – Lcl C-terminal domain D521A mutant Sample: HbP1 (D521A) trimer, 56 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.8 nm Dmax 9.8 nm VolumePorod 108 nm3

SASDUQ7 – Lcl C-terminal domain K526A mutant Sample: HbP1 (K526A) trimer, 56 kDa Legionella pneumophila protein Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29 RgGuinier 2.8 nm Dmax 9.5 nm VolumePorod 104 nm3