Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family

Megalizzi V, Tanina A, Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
Heliyon :e40494 (2024 Nov)

doi: 10.1016/j.heliyon.2024.e40494

Submitted to SASBDB: 2024 May 8 Published in SASBDB: 2024 Nov 18

SASDU88 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - monomeric peak Sample: Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) monomer, 40 kDa Escherichia coli (strain … protein Uncharacterized protein Rv2242 monomer, 43 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH: Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3 RgGuinier 2.8 nm Dmax 8.5 nm VolumePorod 113 nm3

SASDU98 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - dimeric peak Sample: Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) dimer, 80 kDa Escherichia coli (strain … protein Uncharacterized protein Rv2242 dimer, 86 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH: Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3 RgGuinier 3.7 nm Dmax 10.8 nm VolumePorod 345 nm3

SASDUA8 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - tetrameric peak Sample: Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) tetramer, 161 kDa Escherichia coli (strain … protein Uncharacterized protein Rv2242 tetramer, 171 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH: Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3 RgGuinier 5.2 nm Dmax 15.8 nm VolumePorod 826 nm3